ATTN: NOT FOR THE FAINTHEARTED

HELLOW MATES!

had a rat handling workshop yesterday. SEXING THE RAT:

Female and male, it is the difference between the anus and the vagina/penis length

First i learned how to catch a rat, YES by the BASE tail

Next technique: feed rats with my new cocktails.. the nicer term --> gavage.. (forcefeed?)

the CORRECT needle size: from the mouth to the end of ribcage..

Cardiac puncture prior to killing the rat (for post mortem purposes) By the way, cardiac puncture is just a fancy term of saying poking a hole in the heart to drain its blood....

Killing the rat: There are many ways to put it too sleep. It must be done as HUMANE as possible.

1: carbon dioxide poisioning

2: 3X anaesthetic dose

3: General anaesthesia + preceding physical euthanasia (cervical dislocation, decapitation,
exsanguination) --> I had to do a cervical dislocation = breaking its neck.. *

4:Gas anaesthesia overdose

NON acceptable ways of putting a rat down:
• Decompression
• Asphyxiation
• Air embolism
• Freezing (rapid freezing permitted under anaesthesia and with protocol approval)
• Carbon monoxide
• Methoxyflurane
• Ether
• Nitrogen flushing
• Nitrous oxide
• Chloroform
• Chloral hydrate
• Poisons (strychnine, cyanide)
• Household products and solvents (acetone, alcohol)
• Drowning
• Burning
• Physical Methods

HAHAHAHAHA.. some of them are atocious. ANYONE still wants to do research?

kisses..another day...